Open MR-guided brain tumor cryotherapy preliminary study

Title: Open MR-guided brain tumor cryotherapy preliminary studyAuthor: Ji-Qing SongDegree-granting units: Shandong UniversityKey words: magnetic resonance intervention;; rabbit;; animal models;; brain tumor;; cryoablationSummary:Objective To study the MR-guided fine-targeted probes argon-helium cryosurgery of rabbit brain tumor pathology and at different times after high-field magnetic resonance imaging findings, and observe the treatment effect.Methods 26 rabbit brain tumor model, divided into two groups. Treatment group, 20 tumors in rabbits, the line 0.23T open MRI-guided cryoablation therapy, tumor control group, six rabbits, only the line MR-guided implantation of argon-helium refrigeration probe Magnetic lifter within the tumor, no line of cryotherapy. All tumors were in the plant rabbits 6 days after tumor line high-field MRI scans, tumor diameter less than 1.0cm when guided by magnetic resonance Shishi, the use of frozen probe diameter 1.47mm, 40% of the argon gas output power of tumors cryotherapy, freezing 3 minutes, rewarming 1 minute, freeze-thaw cycle twice. Then after 30 minutes, respectively, after 3 days, after 7 days, 14 days after high-field MR scan-line, rabbits were sacrificed 3 tumors, the pathological changes observed. 60 days after treatment, high-field MRI scan line after the last two tumor rabbits were sacrificed, parallel pathological examination. Tumors in the control group were planted tumors in rabbits after 6 days, 10 days-line high-field MRI scans to observe changes in tumor size.The results are cryoprobe - times of successful implantation of the tumor. 20 rabbits treated tumors, the average tumor size before treatment 0.32 ± 0.16 × 0.42 ± 0.18cm, the average size of hockey surgery 0.93 ± 0.03 × 0.93 ± 0.05cm. Six rabbits in the cryotherapy treatment within 24 hours after death. Postoperative MRI showed: Day 3 frozen peri edema, edema on day 7 to day 14 after treatment, frozen tumor edema disappeared, kitchen range and intraoperative http://www.999magnet.com/products/131-magnetic-lifter frozen frozen range shown is similar to the first 60 days after treatment, further reduce the frozen region, showing malacia performance. Postoperative pathology showed: frozen within 30 minutes after the freezing treatment centers see necrotic area, the surrounding area see multifocal hemorrhagic areas and edema of the nerve cells; 3 days after treatment, the center was coagulation necrosis, edema of the surrounding area shows that neurons, blood vessels expand, and lymphocytes; 7 days after treatment, the central necrotic area, surrounded by inflammatory cell infiltration, granulation tissue, and focal hemorrhage areas; frozen 14 days after treatment, granulation tissue, inflammatory cell infiltration ; area 60 days frozen was vacuolization, gliosis, infiltration of lymphocytes and seen a lot of mucus-like cells. 2 rabbit tumors residual tumor after treatment. Were sacrificed on day 60 after treatment, tumors of 2 rabbits, no abnormal symptoms of nervous system damage, pathological examination found no residual tumor. The first two days after the freezing treatment, tumors in rabbits showed irritability, lameness, all in relief three days later and disappeared. Control group, the average survival time of rabbit tumor was 11.17 days.Conclusion: Open MR-guided, using 1.47mm of MR-compatible probes argon-helium can be successfully targeted rabbit brain tumor ablation, cryoablation surgery, coagulation necrosis of tumor after, granulation tissue, gliosis and other procedure cystic and effective.Degree Year: 2009