Study on Toxicity of Rare Earth Ceriun (Ce) to Male Mice Reproduction

Study on Toxicity of Rare Earth Ceriun (Ce) to Male Mice Reproduction
　　"There is petroleum in Middle East; there is the rare earth in China". China is the richest rare earth resource country. The cerium accounts for 28% of the rare earth total matches, occupies the first places, develops and makes use of value highly in all rare-earth elements. With the fact that applicative increasing by , the more and more cerium enter environment, then pass living creature chain, cause the increasing in the living things body and the human body, and its absorption has long-term, so absorption behind in the near future, long-term of the living creature safety becomes a neodymium magnets deserving studied problem, among them to the influence of reproductive system deserve we concern more, because reproductive system is one of the more impressionable system to poison and harmful material. But, currently study is few at home and abroad which concerns on the impact of cerium over reproductive system, therefore the experiment is put into use providing scientific basis of the safety taking by the impact of cerium over male mice reproductive system.1. Accumulation toxicity of cerium to male miceThe experiment adopted a 20d ascending dosage to detect the accumulation toxicity of cerium to male mice.
　　The results showed that: Accumulate modulus was greater than 5.0, the rare-earth element cerium didn’t have notable accumulates toxicity to male mice.2. Asia chronic toxicity of cerium to male mice2.1 Impact of cerium over male mice weightEvery 3d weighted once, and then observed the change of weight to study the impact of cerium over male mice situation during the experiment. The results showed that: In intragastric administration first 20d there was no notable difference between every group; In the first 40d, the average weight of every group was higher than PC group; there was notable difference between NC group and HD group; In the first 60d, the average weight of every group was higher than PC group, there was notable difference between HD group and NC group、LD group, LD group’s average weight was higher than NC group, but there was no notable difference. So it confirmed that to some extent cerium at 15 mg·kg-1, infused 20d can promote male mice weight growth, neodymium magnets but there was no notable difference; at 60 mg·kg-1, infused 40d can inhibit male mice weight growth, effect the male mice situation.2.2 Impact of cerium over male mice testispathological mechanism organizationThe mice were executed by pulled and broken the cervical vertebra, the testis were selected immediately .
　　By HE dyeing, observe morphology change. The results showed that: with doses increasing, epithelium of testis cell separated from basilar part obviously and somniferous tubules epithelial cells were disordered compared with NC group. It confirmed that Ce(NO3)3 can hurt male mice pathological mechanism organization.2.3 Impact of cerium over male mice testis indexThe experiment studied its organic hurt to testiculus by observing an impact of cerium over male mice testis index. The results showed that: there was no notable difference between every group; it showed that the hurt 60d inner cerium brought about to testiculus was not obvious.2.4 Impact of cerium over male mice testiculus cell enzymatic activityEnzyme system is sensitive to poison externally, the sort is changed already when changes happen in other index , especially relevant to energy metabolism enzyme system is easier to be affected. The experiment studied the impact of cerium over male mice reproductive system by neodymium magnets observing LDH、SODH、SDH、G-6PD changes. The results showed that : cerium at 15 mg·kg-1 can promote LDH、G-6PD activity、inhibits SDH activity, but they were both not have notable differences except SODH; during the period of 30 mg·kg-1 dosage had no notable restraint to SODH, but had notable restrain to LDH、SDH、G-6PD; during the period of 60 mg·kg-1 dosage have notable restrain to LDH、SODH、SDH、G-6PD. It confirmed cerium can promote enzymatic activity to some extent in 15 mg·kg-1 (except SDH), but no notable, and restrain enzymatic activity during the period of 60 mg·kg-1.2.5 Impact of cerium over male mice testiculus serum testosterone contents Main physiological action of testiculus is creating sperms; its activities affect function of mesenchyme cell. The results showed that: with contamination dosages increasing male mice serum testosterone contents had down trend, testosterone contents of MD group, HD group and PC group were notable lower than NC group.2.6 Impact of cerium over male mice the cell cycle and apoptosisThe study used One-Fluorescence Dying method on Flow Cytometer to detect the influence of cerium on the cell cycle and apoptosis. The results showed that for G0、G1 phase , cell percentages of HD group and PC group had notable difference compared with NC group, LD group、MD group、HD group had notable difference compared with PC group; for S phase, cell percentages of HD group and PC group had notable difference compared with NC group, LD group had notable difference compared with HD group、PC group; for G2 + M phase and apoptosis , HD group and PC group had notable difference comparing with NC group. It proved that cerium may arouse DNA composing a decrease during the period of 60 mg·kg-1 dosage and the apoptosis increase by.2.7 Impact of cerium over male mice the spermThe experiment studied the impact of cerium over male mice reproductive system by observing sperm’s motility、sperm’s viability、sperm count and teratospermia changes.
　　The results showed that: at 30 mg·kg-1 dosage can decrease viability、sperm count and promote teratospermia, during the period of 60 mg·kg-1 dosage had notable restraint to motility.2.8 Impact of cerium over male mice the reproductive capacityThe experiment studied the impact of cerium over mating rate、pregnancy rate and average live fetus of per litter. The results showed that : mating rate and average live fetus of per litter had descend trend, there was no notable difference between every group; for pregnancy rate, there was also no notable difference between every group. It proved that cerium didn’t have notable impaction over the reproductive capacity of male http://www.chinamagnets.biz/ mice.2.9 Impact of cerium over the fetusThe experiment studied the impact of cerium over female mice’s abnormal embryo rate、fetal death rate and abnormal embryo rate. The results showed that for female mice’s abnormal embryo rate ,HD group and PC group had notable difference comparing with NC group; for fetal death rate every group had promote trend, but only PC group had notable difference comparing with NC group; for abnormal embryo rate, MD group、HD group and PC group had notable difference comparing with NC group. So it showed that cerium can influence the growth and developmental of fetus.CONCLUSION: The experiment about cerium which were touched long time can impact mice’s weight, testicle organization’s pathology , organization’s LDH、SDH and G-6PD, testiculus serum testosterone contents, testicle cell cycle and apoptosis, mice’s sperm. Long time getting in touch with cerium had a certain function to reproduction and genetic toxicity of male mice.