metal complexes of chitosan and its derivatives, the synthesis, characterization and antibacterial properties of cancer

Title: metal complexes of chitosan and its derivatives, the synthesis, characterization and antibacterial properties of cancer
Author: Yang Zifang
Degree-granting units: Shanghai Normal University
Keywords: rare earth;; chitosan;; complexes;; antibacterial agent;; cancer
Summary:
In this paper, chitosan and its derivatives and rare earth and transition metal salts of nitrate as raw materials, the use of reflux condensation and precipitation, a series of new synthesized chitosan and its derivatives, metal complexes CTS / RE (RE = La3, Ce3, Ce4 Pr3, Nd3, Sm3, Eu3, Y3, Er3, Gd3; CTS of chitosan and its derivatives), and by infrared spectroscopy, X-ray photoelectron spectroscopy, X-ray powder diffraction, X-ray energy spectroscopy, Raman spectroscopy, thermogravimetric - differential thermal analysis, transmission electron microscopy, scanning electron microscopy and fluorescence spectra of the complexes of the composition, structure and Strong magnets properties were analyzed. Thermogravimetry - differential thermal analysis results show that the synthesized complexes have better thermal stability. SEM photographs obtained through the analysis of the new complexes are synthesized nanoparticles.
Diffusion method using medium, nutrient broth dilution method, foil method and aging test method for metal complexes of chitosan antibacterial properties, is a representative experiment with E. coli bacteria and Staphylococcus aureus. Antibacterial results show that the synthesized complexes on experiments with bacteria have a better inhibitory, are broad-spectrum antimicrobial agent, has good durability and long-lasting antimicrobial resistance, and glucose complexes against Staphylococcus aureus to antimicrobial capacity greater than the ability of E. coli bacterial. In addition, some complexes in vitro anticancer activity tests, experimental target cell is a human chronic http://www.chinamagnets.biz/ myelogenous leukemia K562 cells. Experiments were applied were inverted light microscope, fluorescent microscope and transmission electron microscopy of K562 cells and the role of the complex morphology of cells after the study, the use of MTT assay for cell viability testing, the use of flow cytometry after the complex role of the withered cell death rate. Experimental results show that the anti-tumor chitooligosaccharides cerium complexes on K562 cell growth and proliferation of tumor significantly inhibited, compared with the control group, significant differences in inhibition rate within a certain range increases with the dose increased.
Degree Year: 2010